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ps100010  (OriGene)


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    Structured Review

    OriGene ps100010
    Ps100010, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 551 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv6+ac+vector/pCMV6-AC-GFP+Mammalian+Expression+Vector/pmc13233774-56-8-5
    Average 96 stars, based on 551 article reviews
    ps100010 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Over Expression:

    Article Title: Mitochondrial Genome-Encoded Long Noncoding RNA Cytochrome B and Mitochondrial Dysfunction in Diabetic Retinopathy.
    Article Snippet: Aims: Mitochondrial dysfunction is closely associated with the development of diabetic complications.. In diabetic retinopathy, electron transport chain is compromised and mitochondrial DNA (mtDNA) is damaged, downregulating transcription of mtDNA-encoded cytochrome B (CYTB) and its antisense long noncoding RNA, long noncoding RNA cytochrome B (LncCytB).. Our goal was to investigate the role of LncCytB in the regulation of CYTB and mitochondrial function in diabetic retinopathy.

    Article Title: Involvement of nitric oxide synthase in matrix metalloproteinase-9- and/or urokinase plasminogen activator receptor-mediated glioma cell migration
    Article Snippet: .. We used uPAR human cDNA cloned in pCMV6-AC vector (Origene, Rockville, MD) for full-length uPAR (U-fl) overexpression. .. U251 human glioma cells obtained from the National Cancer Institute (NCI) (Frederick, MD) were grown in DMEM supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA).

    Article Title: BET inhibition targets ABC-DLBCL constitutive B-cell receptor signaling through PAX5
    Article Snippet: For knockdown, 1 nmol PAX5 Silencer siRNA #s10064 or siRNA negative control #AM4611 (Invitrogen) was electroporated using the preset program DS-104. .. For PAX5 overexpression, the PAX5 plasmid #SC304450 or the pCMV6-AC vector (OriGene, Rockville, MD) was transformed into DH5alpha Escherichia coli cells (OriGene), inoculated overnight, and streaked on lysogeny broth/ampicillin agar plates. .. Isolated clones were inoculated, followed by plasmid isolation using the Plasmid Midi Kit (Qiagen).

    Construct:

    Article Title: Mitochondrial Genome-Encoded Long Noncoding RNA Cytochrome B and Mitochondrial Dysfunction in Diabetic Retinopathy.
    Article Snippet: Aims: Mitochondrial dysfunction is closely associated with the development of diabetic complications.. In diabetic retinopathy, electron transport chain is compromised and mitochondrial DNA (mtDNA) is damaged, downregulating transcription of mtDNA-encoded cytochrome B (CYTB) and its antisense long noncoding RNA, long noncoding RNA cytochrome B (LncCytB).. Our goal was to investigate the role of LncCytB in the regulation of CYTB and mitochondrial function in diabetic retinopathy.

    Article Title: Loss of Mature Lamin A/C Triggers a Shift in Intracellular Metabolic Homeostasis via AMPKα Activation.
    Article Snippet: The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). .. PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-AC vector (PS100020, OriGene) or pCMV6-AC-GFP vector (PS100010, OriGene) to construct plasmids to express lamin A, lamin C and the different mutants. ..

    Clone Assay:

    Article Title: Involvement of nitric oxide synthase in matrix metalloproteinase-9- and/or urokinase plasminogen activator receptor-mediated glioma cell migration
    Article Snippet: .. We used uPAR human cDNA cloned in pCMV6-AC vector (Origene, Rockville, MD) for full-length uPAR (U-fl) overexpression. .. U251 human glioma cells obtained from the National Cancer Institute (NCI) (Frederick, MD) were grown in DMEM supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA).

    Article Title: Loss of Mature Lamin A/C Triggers a Shift in Intracellular Metabolic Homeostasis via AMPKα Activation.
    Article Snippet: The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). .. PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-AC vector (PS100020, OriGene) or pCMV6-AC-GFP vector (PS100010, OriGene) to construct plasmids to express lamin A, lamin C and the different mutants. ..

    Transfection:

    Article Title: Identification of the EBF1/ETS2/KLF2-miR-126-Gene Feed-Forward Loop in Breast Carcinogenesis and Stemness
    Article Snippet: .. Cells were then transfected with 14 μg of either pCMV6-AC empty vector or with EBF1-expressing pCMV6-AC vector (Origene, Rockville, MD, USA), by using Lipofectamine 2000 (Invitrogen), according to manufacturer instructions. ..

    Article Title: Identification of the EBF1/ETS2/KLF2-miR-126-Gene Feed-Forward Loop in Breast Carcinogenesis and Stemness
    Article Snippet: .. Cells were then transfected with 14 μg of either pCMV6-AC empty vector or with EBF1-expressing pCMV6-AC vector (Origene, Rockville, MD, USA), by using Lipofectamine 2000 (Invitrogen), according to manufacturer instructions. ..

    Plasmid Preparation:

    Article Title: BET inhibition targets ABC-DLBCL constitutive B-cell receptor signaling through PAX5
    Article Snippet: For knockdown, 1 nmol PAX5 Silencer siRNA #s10064 or siRNA negative control #AM4611 (Invitrogen) was electroporated using the preset program DS-104. .. For PAX5 overexpression, the PAX5 plasmid #SC304450 or the pCMV6-AC vector (OriGene, Rockville, MD) was transformed into DH5alpha Escherichia coli cells (OriGene), inoculated overnight, and streaked on lysogeny broth/ampicillin agar plates. .. Isolated clones were inoculated, followed by plasmid isolation using the Plasmid Midi Kit (Qiagen).

    Article Title: Loss of Mature Lamin A/C Triggers a Shift in Intracellular Metabolic Homeostasis via AMPKα Activation.
    Article Snippet: The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). .. PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-AC vector (PS100020, OriGene) or pCMV6-AC-GFP vector (PS100010, OriGene) to construct plasmids to express lamin A, lamin C and the different mutants. ..

    Transformation Assay:

    Article Title: BET inhibition targets ABC-DLBCL constitutive B-cell receptor signaling through PAX5
    Article Snippet: For knockdown, 1 nmol PAX5 Silencer siRNA #s10064 or siRNA negative control #AM4611 (Invitrogen) was electroporated using the preset program DS-104. .. For PAX5 overexpression, the PAX5 plasmid #SC304450 or the pCMV6-AC vector (OriGene, Rockville, MD) was transformed into DH5alpha Escherichia coli cells (OriGene), inoculated overnight, and streaked on lysogeny broth/ampicillin agar plates. .. Isolated clones were inoculated, followed by plasmid isolation using the Plasmid Midi Kit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Loss of Mature Lamin A/C Triggers a Shift in Intracellular Metabolic Homeostasis via AMPKα Activation.
    Article Snippet: The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). .. PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-AC vector (PS100020, OriGene) or pCMV6-AC-GFP vector (PS100010, OriGene) to construct plasmids to express lamin A, lamin C and the different mutants. ..

    Purification:

    Article Title: Loss of Mature Lamin A/C Triggers a Shift in Intracellular Metabolic Homeostasis via AMPKα Activation.
    Article Snippet: The untagged human LMNA (lamin C) (NM_005572) clone (#SC321549) and the tagged human LMNA (lamin A) (NM_170707) Clone (#RC204970) were purchased from OriGene Technologies (Jiangsu, China). .. PCR products containing the LMNA/C cDNA or different mutations were purified, sequenced (Rui Bo Xing Ke Company, Beijing, China) and then cloned into the pCMV6-AC vector (PS100020, OriGene) or pCMV6-AC-GFP vector (PS100010, OriGene) to construct plasmids to express lamin A, lamin C and the different mutants. ..



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